Histolytica life cycle consists of an infective cyst and an invasive trophozoite form. Cysts are typically found in formed stool whereas trophozoites are typically found in diarrheal stool.
Amebiasis is an infection caused by a parasite known as entamoeba histolytica.
Entamoeba histolytica cyst in stool analysis. Histolytica infections occur when cysts are ingested through contaminated food or water. It has been estimated that 40 million to 50 million people develop clinical amoebiasis each year resulting in up to 100000 deaths 15. As a result of unknown stimuli in the intestine trophozoites again can differentiate into cysts a process known as encystation.
Infection with entamoeba histolytica and edispar occurs via ingestion of mature cysts from fecally contaminated food water or hands. The main purpose of detection and differentiation of entamoeba species in stool samples is the detection of the causative agent of amoebic dysentery entamoeba histolytica. Signs and symptoms include stomach pain and cramping and loose stools.
Stool examination is the method of choice for recovering entamoeba coli trophozoites and cystsalthough not considered pathogenic the presence of entamoeba coli suggests ingestion of contaminated food or drinktherefore in addition to the non pathogenic entamoeba coli laboratory technicians should carefully examine these preparations for the presence of pathogenic parasites. Exposure to infectious cysts and trophozoites in fecal matter during sexual contact may also occur. The quadrinucleate cyst is resistant to gastric acidity and desiccation and can survive in a moist environment for several weeks.
In the lower intestine trophozoites emerge from cysts a process known as excystation. Only the trophozoite form invades human tissue.
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